Regulatory

Part:BBa_K4115018:Design

Designed by: Kaijun Wang   Group: iGEM22_ShanghaiTech_China   (2022-09-25)


PyciG, a stress induced promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The native RBS is contained in this part, so you don't need to add your own RBS while using it.

Source

We amplified this part from the E.coli DH5-alpha genome.

References

[1] Rozen, Y., Dyk, T.K., LaRossa, R.A., and Belkin, S. (2001). Seawater Activation of Escherichia coli Gene Promoter Elements: Dominance of rpoS Control. Microb Ecol 42, 635-643. 10.1007/s00248-001-1012-x.
[2] Sunya, S., Delvigne, F., Uribelarrea, J.L., Molina-Jouve, C., and Gorret, N. (2012). Comparison of the transient responses of Escherichia coli to a glucose pulse of various intensities. Appl Microbiol Biotechnol 95, 1021-1034. 10.1007/s00253-012-3938-y.